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article · Sahel Journal of Life Sciences FUDMA

Protein Quality and Haematological Profile of Rats (Albino) Fed with Malted and Fermented Maize Based Complementary Food Supplemented with Soybean and Orange Fleshed Sweet Potato Flour

2025Open accessBenue State University

In plain language

This study evaluated the protein quality and haematological safety of complementary food blends made from malted and fermented maize supplemented with soybean and orange-fleshed sweet potato flours. Researchers developed four composite formulations and tested them alongside an unfortified maize control and a commercial infant cereal over a 28-day feeding trial in albino rats. Growth metrics and blood markers associated with liver and kidney function were monitored to assess nutritional suitability and safety. Rats fed the commercial control achieved the highest weight gain and protein efficiency ratio, while the unfortified maize performed lowest across these performance metrics. Across all tested groups, blood parameters including packed cell volume, red and white blood cell counts, and haemoglobin levels remained within normal ranges. The evaluated food blends showed no signs of organ impairment, indicating their potential suitability as nutritious complementary diets.

Key takeaways

  • Weight gain in rats ranged from 32.67 grams for unfortified maize to 230.67 grams for the commercial cereal control.
  • Protein efficiency ratios ranged between 0.45 for unfortified maize and 2.47 for the commercial cereal control.
  • Packed cell volume spanned from 33.67 percent in the commercial control group to 40.67 percent in the fermented composite flour group.
  • Haematological assessments showed that the formulated complementary blends caused no organ impairment in the tested animals.

Why it matters

Developing nutritious, locally sourced complementary foods is vital for addressing infant and child malnutrition. Testing composite blends of maize, soybean, and orange-fleshed sweet potato in animal models provides crucial evidence on whether such formulations can support healthy growth and maintain normal blood and organ health without toxic side effects.

Commercialisation angle

This research could inform the formulation of affordable, fortified complementary foods and infant cereals by food manufacturers or public health nutrition programmes. The findings reflect early-stage in vivo testing in animal models. Extensive clinical trials in target human populations, processing standardisation, and sensory evaluations would be necessary before these formulations can advance to commercial production.

AI-generated from the published abstract. Always read the original work before citing.

Abstract

This study investigated the protein quality and haematological profile in rats fed malted and fermented maize-based complementary food supplemented with soybean and orange-fleshed sweet potato composite flours. Flours were prepared from malted and germinated maize grains and supplemented with soybeans and orange-fleshed sweet potato flour as the complementary foods. The combination of the flours was obtained by material balancing. Four food formulations were obtained and named NMSS, FMSS, MFMSS, and MMSS, NM(control) and Nestle Cerelac (commercial control). Twenty-four Wistar strain of albino rats were grouped into six groups of four rats per cage and were fed the formulated blends and control. The rats were fed for 28 days. Weight changes and haematological parameters of the kidney and liver were examined to assess the suitability of these formulated diets. ANOVA was used for statistical analysis. The results of the total weight gain ranged between 230.67 and 32.67 g in Cerelac and NM, respectively. PER ranges from 2.47 (cerelac) – 0.45 (NM), and FCE ranges from 15.04 (NM) – 2.62 (cerelac). The haematological parameters analysis revealed that PCV ranges between 40.67 (FMSS) and 33.67 % (Cerelac), RBC ranges from 5.50 to 4.60 X103mm3, HGB ranges from 12.22 to 11.00 g/dl, WBC ranges from 5.93 to 5.13 X103mm3, Lymphocytes ranges from 35.67 to 34.33 %, Neutrophil ranges from 58.00 to 56.00 % and Eosinophil ranges from 3.00 to 2.33 %. The results showed that the formulated blends did not impair any significant organ of the rats, as indicated by the haematological studies.

Research topics

  • GABA and Rice Research
  • Natural Antidiabetic Agents Studies
  • Food and Agricultural Sciences

Read the original research

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DOI: 10.33003/sajols-2025-0302-40

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