Tissue Friendly Dentine Treatments (In Vitro Study)

Abstract

Non biocompatible Irrigants will cause disintegration of cell proliferation, adhesion and enzyme systems in the area of contact and cause periodontal tissue irritation, damage and delayed wound healing. Human Periodontal Ligament Fibroblast cells are the first cells that face the irrigation solutions periapically. In a search for a stem cell friendly irrigation, this study was designed to compare sodium hypochlorite to HEDP Dual rinse Solution and Curcumin. The study is registered (FD BUE REC 21-032). It encompass two parts using 20 days confluent stem cells: Part I Determination of sample cytotoxicity on cells:. At the established times, media was mixed with MTT solution. Optical density at 560nm was read and subtracted from background at 620nm, using Spectrophotometer. Part II Determination of cell proliferation: At the established times, cells were harvested, stained with 0.1 ml of trypan blue solution in buffer and total number of cells and number of viable cells were counted by hemocytometer: Data were statistically analyzed for normality. Homogeneity of variances was tested using Levene's test followed by one-way ANOVA and Tukey's post hoc test. HEDP recorded best results in the study. At 1 and 5 minutes group (I) HEDP recorded the best value in MTT assay followed by group (II) Curcumin. Trypan blue staining showed A significant difference between all irrigation solutions at all observation periods. The best value was found in HEDP followed by Curcumin in all periods. Dual Rinse HEDP and curcumin are tissue friendly and cause less toxicity than standard irrigant.