Therapeutic Potential of Bee Venom ( Apis mellifera intermissa ) in Reversing Aniline-Induced Acute Anaemia and Spleen Oxidative Damage in Mice

Abstract

Objectives: ) from north-eastern Algeria in mitigating these effects. Methods: Acute anemia was induced via oral administration of aniline hydrochloride (100 mg/kg body weight), while recovery was assessed following intraperitoneal injection of bee venom (0.76 mg/kg body weight) for six days. Results: ), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), index of distribution of RBC (IDR), and index of distribution of platelets (IDP), along with increased malondialdehyde (MDA) levels, and decreased reduced glutathione (GSH) level and catalase activity in spleen tissues. Histopathological analysis revealed splenomegaly, hyperplasia of the white pulp, and increased extramedullary hematopoiesis. Bee venom treatment significantly reversed these effects, restoring hematological parameters, antioxidant enzyme levels, and spleen architecture. Conclusion: Bee venom showed potential as a natural therapeutic agent against aniline-induced anaemia and oxidative spleen damage, highlighting its antioxidative and restorative properties.