The role of IFN-γ + 874T/A and IL-12 + 16974A/C polymorphisms in susceptibility to tuberculosis infection in a Sudanese population: a case-control study

Abstract

BACKGROUND: Tuberculosis (TB) is a top infectious disease killer worldwide. The susceptibility to infectious diseases, including tuberculosis, has been linked to several cytokine gene polymorphisms. METHOD: A case-control study was conducted at Abu Anja Chest Hospital, Omdurman, Khartoum state, using PCR-RFLP in 200 Sudanese patients with pulmonary tuberculosis and 200 healthy controls between February 2018 and February 2021. Data analysis was done using the IBM SPSS software package version 20.0. (Armonk, NY: IBM Corp). RESULT: The mutant and heterozygous genotypes of IL-12+16974 A/C polymorphism were associated with an almost four-fold [P-value <0.001 OR= 3.703(2.243- 6.115), 95% CI] increased risk of TB in the Sudanese population. No statistically significant differences were found in the genotype and allele frequencies of the IFN-γ+874T/A gene between patients and controls. CONCLUSION: These findings suggest, for the first time in Sudan, the significant risk of TB in people carrying the mutant and heterozygous genotypes of IL-12+16974 A/C genes.