The Potential Effect of Autologous Injectable Platelet Rich Fibrin (I-PRF) on Healing of Experimentally Induced Full-Thickness Skin Wound in Donkeys

Abstract

The aim of this study is to evaluate the potential effect of autologous I-PRF on healing of experimentally induced full-thickness skin wound in donkeys. Six adult healthy donkeys were subjected to two full thickness skin wounds on the back region. Hence, 2 groups were evaluated; control group (wounds at the left side) treated with local daily dressing with povidone iodine and I-PRF group (wounds at the right side) treated with I-PRF solution at day 0 and repeated at day 7 PW. Wounds were clinically evaluated for determination of the wound surface area, percentages of wound area and contraction. Histopathological evaluation was performed at days 21 and 42PW. Immunohistochemical evaluation was performed at 21 days PW for detection of CD31 and alpha smooth muscle actin (α-SMA) markers. The wound surface area was significantly lower in I-PRF group than the control group from 7 up to 42 days post wounding, the wound contraction percentage was significantly higher in I-PRF group than the control group from 14 up to 42 days PW. Better histopathological findings were demonstrated in I-PRF group compared to control group. Immunohistochemical evaluation revealed high immunreactivity against both CD31 and α-SMA antigens in I-PRF group and low immunreactivity against CD31 antigen and no immunreactivity against α-SMA antigen in control group. In conclusion, I-PRF improvesthe healing of experimentally induced full thickness skin wounds in donkeys evidenced by faster wound contraction as well as well-formed granulation tissue with complete re- epithelization.