article · Aquaculture Reports
Zebrafish were fed diets supplemented with the probiotic bacterium Pediococcus acidilactici MA 18/5 M at three different concentrations over an eight-week trial to evaluate metabolic enzymes and gene expression related to growth, immunity, and antioxidant function. Core metabolic enzymes, including creatine phosphokinase, lactate dehydrogenase, alkaline phosphatase, and transaminases, showed no significant changes across all groups. However, the probiotic feed enhanced digestive protease activity. Lysozyme activity rose in fish receiving medium and high doses, though lysozyme gene expression increased solely at the highest concentration. Furthermore, the lowest probiotic dose increased the expression of genes associated with growth hormone, tumour necrosis factor, insulin-like growth factor, and catalase. Conversely, glutathione S-reductase expression fell at higher supplementation levels. Overall, dietary inclusion of this probiotic supported growth and immune status in the fish.
Identifying dietary supplements that enhance fish health without causing metabolic stress is valuable for sustainable aquatic farming and aquatic biology. By demonstrating improvements in digestive enzyme activity alongside positive immune and growth gene responses, this work shows how specific probiotic dosages can be targeted to support animal welfare and physiological resilience in controlled aquatic environments.
This work points towards applications in aquafeed manufacturing, specifically the formulation of probiotic functional feeds to boost growth and immune readiness in fish. The potential end users are commercial fish farms, feed producers, and ornamental fish keepers. Given that the trial was conducted strictly in a laboratory setting using zebrafish, the research is at an early experimental stage and requires field trials in commercial aquaculture species.
AI-generated from the published abstract. Always read the original work before citing.
This study investigated the influence of probiotic Pediococcus acidilactici MA 18/5 M on the metabolic enzyme activities and mRNA levels of growth, immunity, and antioxidative-related genes in zebrafish. Zebrafish fed with diets containing P. acidilactici MA 18/5 M at 0, 107, 108, and 109 CFU/g levels for eight weeks. The obtained results revealed that the creatine phosphokinase, lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase remained unchanged in all groups (P > 0.05). Dietary P. acidilactici enhanced protease activity relative to the control group (P < 0.05). Compared with the non-treated group, lysozyme activity increased in fish fed with high and medium doses of P. acidilactici, but the mRNA level of lysozyme gene was only up-regulated in the fish received the high dose of P. acidilactici (P < 0.05). Meanwhile, gene expressions of IGF-1, TNF-α, GH-1, and catalase were increased by P. acidilactici at 107 CFU/g relative to the control group. Conversely, the expression of glutathione S-reductase (GSR) decreased in zebrafish fed with medium and high doses of P. acidilactici compared to the control group. Thus, dietary administration of P. acidilactici MA 18/5 M, especially at 107 CFU/g diet, could improve the growth, immunity, and health status of zebrafish.
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DOI: 10.1016/j.aqrep.2020.100374
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