The effect of methylene blue on water hardness and antibacterial activity and the environment

Abstract

This research was carried out to compare complexometric titration methods for the determination of water hardness in the presence of two colored indicators. The complexometric titration with EDTA method has evolved from the typical method of determining water hardness rates used in various references in several countries. Our objective is to make a comparison between Methylene Blue (MB) and Eriochrome black T (EBT) used as color indicators to determine the hardness in drinking water as a function of the flow. Several chemical – physic were used to determine the maximum absorbance of the two dyes UV-visible, identification of functional groups by infrared spectroscopy (FT-IR), supported by the effect of concentration conductivity, pH and turbidity and density. Test results to determine water hydrometric title (TH) in the presence of two colored indicators indicated that the equivalent volume values (Veq) are doubled for the BM indicator compared to the EBT indicator however with impressive TH values in two different intervals between 10 < TH< 20 and 30 < TH < 50. This shows that the colored indicator Methylene Blue has important differences. An antibacterial activity is observed on the dye by the disk method on four bacteria in the agar culture medium, from which it can be said that there is sensitivity to antimicrobial agents against methylene blue.