Seroprevalence of Human Papilloma Virus 6,11,16 and 18 among pregnant women in Mwanza-Tanzania

Abstract

<title>Abstract</title> Introduction: High-risk human-papilloma viruses 16 and 18 (HR-HPV 16 and HR-HPV-18) are well known to be associated with carcinoma of the cervix, head and neck, penis, and anus. Low-risk human papillomaviruses 6 and 11 (LR-HPV 6 and LR 11) infection has been associated with anogenital warts, oral papilloma, and laryngeal papillomatosis in children. HPV infection during pregnancy (HR-HPV and LR-HPV) increases the risk of vertical transmission from infected pregnant women to unborn children. The burden of HR-HPV type 16 and 18 and LR-HPV 6 and 11 is not well documented among pregnant women attending antenatal clinics (ANC). This study determined the seroprevalence and distributions of HR-HPV 16, 18, and LR –HPV 6, 11 antibodies among pregnant women attending ANC at BMC in Mwanza, Tanzania. Methodology: A cross-sectional study involving 255 pregnant women enrolled in obstetrics and gynecology outpatient clinics was conducted between November 2020 and March 2021 at Bugando Medical Centre (BMC) in Mwanza. A structured pre-tested questionnaire was used to obtain patients' information. Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) was used for quantitative detection of immunoglobulin G (IgG) from sera. Stata version 15v1 was used for data analysis. Results The median age was 27(IQR: 22–31) years. The overall HPV seropositivity was 63.9% (165/255), 95% CI: 58.0-69.7, whereby 37.6%( 97/255), 32.2%( 83/255), 15.5% (40/255) and 27.1% (70) were positive for HPV 6, 11, 16 and 18 respectively. Eight participants (3.1%) were positive for all 4 genotypes. Conclusion About two-thirds of pregnant women had antibodies against HPV 6, 11 16, and 18 indicating previous exposure to this virus. Vaccination programs should be emphasized to reduce the HPV-related manifestations in this population.