Screening of some actinomycetes isolates for their ability to biosynthesize collagen nanoparticles, optimization of fermentation conditions for improved biosynthesis and anti-hemolytic activity

Abstract

Collagen nanoparticles (collagen-NPs) have several advantages over other natural and synthetic polymeric NPs, including suitable biocompatibility and biodegradability, low antigenicity, a high contact surface, and reduced toxicity. Collagen-NPs appropriate for use in the field of biomedicine in skin wound healing, tissue engineering, drug delivery, in bone grafting, and healing as they promote optimal bone regrowth and for treatment of the damaged nerve tissues and nerve regeneration. Eight strains were assessed for their abilities for the biosynthesis of collagen-NPs using their cell-free supernatant. After incubation, white turbidity appeared as the result of collagen-NPs formation. The most potent strains for maximum biosynthesis of collagen-NPs were identified as Streptomyces xinghaiensis strain NEAA-1 and Streptomyces plicatus strain NEAA-3. These Streptomyces strains were tested for collagen-NPs biosynthesis under different conditions, such as concentrations of collagen and strains cell-free supernatant, temperature, initial pH, and incubation time. The maximum biosynthesis of the collagen-NPs was 4.13 mg/mL and 5.16 mg/mL, respectively, at 35 ºC and 40 ºC for Streptomyces xinghaiensis and Streptomyces plicatus. The maximum biosynthesis of the collagen-NPs was 5.24 mg/mL and 6.33 mg/mL, respectively, at 7 pH for Streptomyces xinghaiensis and Streptomyces plicatus. The maximum biosynthesis of the collagen-NPs was 6.51 mg/mL and 7.42 mg/mL after 48 h for Streptomyces xinghaiensis and Streptomyces plicatus, respectively. The biosynthesized collagen-NPs showed 96% anti-hemolytic activity.