Scanning electron microscopy and morphometric analysis of the hair in dromedaries with SEM-EDX in relation to age

Abstract

BACKGROUND: Hair characterization is critical for determining animal individuality throughout life. This study aimed to assess the morphological features of dromedary camel hair in relation to age. MATERIALS AND METHODS: Hair samples were obtained from the camel humps of 30 dromedary camels separated into three groups: G1 (n:10) aged one-year, G2 (n:10) aged 3-5 years, and G3 (n:10) at the age of 8-10 years. The hair was examined using light microscopy, SEM, and SEM-EDX. RESULTS: The Maghrebi camel had varied medulla patterns and structures across the ages. In the G1 group 75% had continuous medulla patterns and amorphous medulla structures, compared to 70% in G2, and 90% in G3. The medulla index increased with age, rising from 0.3 to 0.77%. The shaft width grew in size from G1 to G2, then fell to approximately one-third of the G2 size at G3. The cortex and cuticle widths were also determined by age, and they increased in the G1 compared to G3 camels. The shape of the cuticle scales in G1 camels was wavy, like mountain tops with irregular edges, within G2 camels the scales were particularly long, oval-shaped scales with smooth, wavy borders. The scales of the older G3 camels were quite long and rectangular. SEM-EDX spectra recognized carbon, oxygen, nitrogen, sulfur, calcium, aluminum, silicon, and potassium at the medulla and cortex. Sulfur levels were highest in the G2 samples but lowest in the G1 samples. CONCLUSION: The dromedary camel's hair structure and mineral content, particularly carbon and nitrogen, differed as camels aged.