Role of Tumor Necrosis Factor Alpha Gene Polymorphism (TNFα -308 G/A) and Prediction of Diabetic Nephropathy

Abstract

Background: Diabetic nephropathy, a chronic kidney disease resulting from diabetes mellitus microvascular complications, is thought to be the primary cause of end-stage renal failure. Gene mutations affecting the TNF gene can impact the generation of tumor necrosis factor-α (TNF-α), which has been associated with worse clinical outcomes in patients with diabetic nephropathy who are in severe condition. Objective: The purpose of this study was to assess the relationship between diabetic nephropathy and TNFα -308 G/A gene polymorphism in individuals with diabetes. Methodology: 50 diabetic individuals were recruited in this case-control research and were split into two groups: 25 patients with nephropathy and 25 patients without nephropathy. As a typical control group, a third set of blood donors (n = 25) appeared to be in good condition. Patients' information was gathered through the use of an interview questionnaire. The test for gene polymorphism was PCR. The TNF α308 G/A gene's A allele has a positive, statistically significant correlation (P-value ~0.05) with DN. Results: The prevalence of DN among the study participants may be predicted by the presence of an A allele in the TNF α308 G/A gene with a statistically significant P-value (P-value < 0.05). The study participants' TNF α308 G/A gene patterns and albumin/creatinine ratio did not exhibit a statistically significant relationship. Conclusion: According to this study, having an A allele in the TNF α308 G/A gene may contribute to the pathophysiology of diabetic nephropathy and be a predictor of nephropathy in diabetic populations.