Role of CD200 as diagnostic and prognostic marker for chronic lymphocytic leukemia

Abstract

Introduction B-cell chronic lymphoproliferative disorders (B-CLPDs) include different diseases derived from clonal expansion of mature B cells. Differentiating B-CLPDs from each other is of critical importance because of different courses, outcomes, and treatment options. This study was done to show the diagnostic and prognostic role of CD200 in chronic lymphocytic leukemia (CLL) patients. Patients and methods The study included 100 patients with B-CLPD whose samples were referred to flow cytometry laboratory, South Egypt Cancer Institute (SECI). CD200 expression on mature B-cell neoplasm was studied by eight color flow cytometry (FACS CANTO 11, Serial number V33896201978; three lasers, eight color flow cytometry) in combination with lymphoma panel markers. Results CD200 shows significant sensitivity (100%), considerable accuracy (78%), and specificity of (26.7%) as a diagnostic marker for CLL. Strong correlation with statistical significance was observed between Rai stages and mean fluorescence intensity of CD200, also increased mean fluorescence intensity of CD200 was associated with positivity for CD38. Conclusion Expression of CD200 plays an important role in the diagnosis and differential diagnosis of different types of B-CLPDs especially in CLL cases. It also may have prognostic role in cases of CLL.