Reviewer response for version 1

Abstract

Cutibacterium acnes is a bacterial skin commensal that is often isolated during routine testing of blood products like platelet concentrates (PCs). Due to the slow growing nature of this bacterium in culture media, C. acnes contaminated PCs are often transfused into vulnerable patients before retrieval of these units can be initiated. This study aimed at obtaining the whole genome sequence of six C. acnes isolates derived from contaminated PCs and to phylotype, compare, and assess their genetic backgrounds. Furthermore, the whole genomes of the PC isolates were compared to clinical isolates obtained from different sites and types of infection. The results indicate that the PC isolates assessed belonged to four phylotypes namely: IA, IB, II and III. Whole genome comparisons identified differences in the virulence profiles of the isolates, providing insight into the potential enhanced risk to patients transfused with C. acnes contaminated PCs.