article · Egyptian Journal of Biological Pest Control
Identifying specific fungal species from natural environments is critical for studying and using beneficial organisms in farming. Researchers developed sequence-characterised amplified region markers to rapidly detect two distinct fungi, Trichoderma koningiopsis and Trichoderma longibrachiatum. Using twenty inter-simple sequence repeat primers, specific diagnostic markers were isolated that amplify a single unique DNA band for each targeted species while remaining absent in other Trichoderma strains. Genetic analysis confirmed that these specific DNA fragments show no meaningful sequence homology with previously catalogued sequences in the National Center for Biotechnology Information and TrichOKEY databases. Compared to conventional identification methods, these new molecular markers provide a faster and simpler mechanism to track fungal population dynamics and confirm the establishment of these fungi following release into agricultural settings.
Beneficial fungi are often released into fields to support crops, but tracking their presence among other native microbes is technically demanding and slow. Having precise molecular markers makes it easier to verify that released strains survive and establish themselves. This capability supports both ecological research and the practical management of biological agents used in sustainable agricultural systems.
This work enables diagnostic monitoring for agricultural producers, agronomists, and biocontrol manufacturers wishing to confirm the presence and persistence of specific Trichoderma strains after field application. The research is at an applied laboratory testing stage, having validated specific molecular targets. Commercial adoption would require formulating these diagnostic markers into standardised, field-ready or commercial laboratory testing kits for quality control and field efficacy monitoring.
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Molecular markers enable the detection and classification of fungi isolated from their natural environments. To develop species-specific markers for detecting Trichoderma koningiopsis and T. longibrachiatum, the sequence-characterized amplified region technique, using 20 inter-simple sequence repeat-polymerase chain reaction primers, was performed. The two specific markers for amplifying a single unique band consistent with T. koningiopsis and T. longibrachiatum, which were absent with other Trichoderma strains, were successfully identified. These fragments had no meaningful sequence homology with known sequences available in the National Center for Biotechnology Information and TrichOKEY databases. Compared with traditional identification techniques, these markers can facilitate more rapid and less complicated studies of Trichoderma population dynamics and evaluate their establishment after release into agricultural environments.
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DOI: 10.1186/s41938-019-0113-0
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