Prevalence of Pseudomonas and Vibrio Species in Shellfish with Special Reference to Their Susceptibility to Antibacterial Agents, Toxigenic and Virulent Genes

Abstract

250 samples were randomly taken from the Port-Said markets, 125 from each of the seemingly healthy Om EL-Khloul "Donax trunculus anatinus" and Baclawese "Tartufo di mare" samples. and subjected to bacteriologically, sensitivity to some antibacterial agents and molecular examination. The prevalence of Pseudomonas and Vibrio species from the examined Om EL-Khloul samples were 35/125 (28.00%) and 30/125 (24.00%) respectively, while that of Baclawese were 52/125 (41.60%) and 40/125 (32.00%) respectively. Biochemical and API identification of the isolates revealed that Pseudomonas species were identified as P. aeruginosa, P. putida, and P. florescent. In contrast, Vibrio species were V. parahaemolyticus, V. vulanificus, V. anguillarum, V. alginolyticus, and V. metschnikovil. Serological typing of P. aeruginosa were P. aeruginosa polyvalent III Group E and P. aeruginosa polyvalent III Group F. while V. parahaemolyticus were identified as O3:K6, O4:K68, O4:K6, O1:K25 and O11:K36. Pseudomonas serovar resists the most studied antibacterial except gentamicin, doxycycline, and tobramycin. Most Vibrio serovar resists studied antibacterial but are sensitive to a lesser extent to Amoxicillin, Chloramphenicol, and Norfloxacin. The molecular study showed that 16S rRNA was detected in Pseudomonas and Vibrio species. A study of the virulence and toxigenic genes showed that toxA and oprL genes were detected in Pseudomonas aeruginosa and the recA genes in Vibrio parahaemolyticus.