Prevalence and Resistance Pattern of Enterococcus faecalis in Cultured Oreochromis niloticus at Ismailia Governorate, Egypt

Abstract

The present study aimed to investigate the prevalence and antibiogram pattern of E. faecalis isolated from 100 O. niloticus showed opaque eye, hemorrhage, dark pigmentation in the whole body, distended abdomen, hemorrhage, and ulceration of skin. Gills, skin, kidney, and spleen from each fish were collected for bacteriological findings.The samples were collected randomly from May to October 2022 from private fish farms located in Ismailia Government. The obtained pinpoint white colonies were confirmed biochemically and tested against different antimicrobial agents by disc diffusion assay. All isolates were examined for 16SrRNA sequence by PCR. The prevalence of E. faecalis in all examined organs were 69/100 (69%). The Gills showed high prevalence rate of infection among the other collected organs, Followed by liver, kidney and spleen with a percentage (28.9%, 26.7%, 24.1%, and 20.3%),respectively. The recovered isolates showed resistance to ampicillin, tetracycline and erythromycin. Isolates were sensitive to linezolid, imipenem and levofloxacin.