PHYLOGENETIC ANALYSIS OF BOVINE EPHEMERAL FEVER VIRUS ISOLATED FROM EGYPTIAN OUTBREAKSIN (2013 TO 2016)

Abstract

Bovine ephemeral fever(BEF) suspected outbreaks recorded during the summer of (2013 to 2016)in the Delta provinces of Egypt. The aim of this study was the isolation and molecular characterization of BEF viruses detected from these outbreaks, as there is no currently information about BEFV in Egypt. Blood samples were collected from suspected cases andthe virus was isolated intracerebrallyin baby mice then passed to Madin Darby Bovine Kidney (MDBK) andAfrican green monkey kidney (VERO) cell lines. The isolated virus was identified by RT-PCR followed by sequencing of the glycoprotein (G) gene of BEFV isolate.The construction of phylogenetic tree based on genetic relationships of the BEFV isolate derived from Egypt and different countries was performed. The phylogenetic treeshowed that the Egyptian isolate was closely linked to the Israeli and Turkish isolates.