Phthalocyanine based metal organic frameworks for electrochemical detection of human epidermal growth factor receptor 2

Abstract

Herein, we report on the development of a phthalocynaine based metal organic frameworks (MOF) for the detection of human epidermal growth factor receptor 2 (HER2). Phthalocyanines (Pcs) exhibit good redox properties, hence their utilization as precursors for the synthesis of Pc based MOFs. The successful preparation of the MOF was confirmed using X-ray powder diffraction (XRD), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), and Brunner Emmet Teller (BET) analysis. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were utilized for electrochemical characterization of the Co octacarboxy Pc (CoOCPc), and Co-CoOCPc-MOF modified glassy carbon electrode (GCE). Differential pulse votammetry was employed for detection of HER2, which is a biomarker for cancer. The selectivity towards HER2 biomarker was accomplished by attaching an aptamer (Apt) onto the MOF modified glassy carbon surface. The GCE/Co-CoOCPc-MOF/Nf/Apt (Nf = Nafion) showed excellent analytical parameters with lowest limit of detection of 5.4 × 10 ng/mL, good repeatability and stability.