article · Heliyon
Silver nanoparticles can be synthesised through a green method using aqueous leaf extract from Annona muricata. The resulting crystalline particles feature a face-centred cubic phase, an average distribution size of 35 nanometres, and a negative surface charge. In laboratory evaluations, the bio-fabricated nanoparticles exhibited strong antioxidant effects by scavenging free radicals and suppressing lipid peroxidation in a dose-dependent manner. In addition, the particles demonstrated anti-diabetic potential by inhibiting key digestive enzymes, namely alpha-amylase and alpha-glucosidase. Testing also confirmed effective antimicrobial activity across several bacterial strains alongside cytotoxic effects in human keratinocyte cell cultures. Overall, the plant-mediated approach yields stable nanoparticles with broad bioactivity relevant to therapeutic investigations.
Using plant extracts to manufacture silver nanoparticles offers an alternative green synthesis route that avoids hazardous chemicals. Demonstrating multiple biological effects, including antioxidant, antimicrobial, and enzyme-inhibiting activities, helps identify sustainable materials that could address microbial resistance and metabolic disorders. Understanding these multifaceted properties is an important baseline for creating bio-compatible interventions in healthcare and personal care.
This research is at an early laboratory stage and is relevant to developers of antimicrobial agents, topical formulations, and metabolic health therapeutics. The results indicate that Annona muricata-derived nanoparticles could support new antibacterial treatments or anti-diabetic formulations. However, because tests were limited to in vitro assays and cell lines, significant pre-clinical safety validation and mechanistic studies remain necessary before any commercial development can occur.
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Green synthesis of metal nanoparticles is reputed to have a robust range of biomedical applications. Silver nanoparticles (AgNPs) bio-fabricated using aqueous leaf extract of Annona muricata were characterized and evaluated for in-vitro antioxidant, lipid peroxidation inhibition, anti-diabetic and antimicrobial activities as well as cytotoxicity in human keratinocyte cells (HaCaT). The extract induced colour change of silver salt solution which absorbed at 420 nm and confirmed the formation of AgNPs. FTIR showed that free amide and hydroxyl groups were responsible for the synthesized nanoparticles. Both XRD and SAED confirmed the crystalline nature of the particles with face centered cubic (FCC) phase. The zeta potential revealed -27.2 mV potential and average distribution size of 35 nm. DLS indicated that the majority of the particles were 86.78 nm size and with a polydispersity index (PDI) of 0.329. AgNPs displayed strong activities against DPPH (IC 50 = 51.80 μg/ml), ABTS (IC 50 = 30.78 μg/ml), α-amylase (IC 50 = 0.90 μg/ml) and α-glucosidase (IC 50 = 3.32 μg/ml). The particles exhibited a dose-dependent inhibition of Fe 2+ -induced lipid peroxidation with effective antimicrobial activity against a battery of bacterial strains and cytotoxicity in HaCaT cell line. These findings revealed the potential biomedical applications of the particles and further work will be required to establish its molecular mechanism of action.
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DOI: 10.1016/j.heliyon.2020.e05413
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