PHENOTYPIC AND GENOTYPING CHARACTERIZATION OF PASTEURELLA MULTOCIDA IN FARM ANIMALS

Abstract

Pasteurellamultocida which is the common cause of respiratory disease in cattle causing higheconomic losses. To achieve this objective, this study succeeded in isolating a total of27 of P. multocida isolates from 280 samples (9.6%). Lung tissues showed the highestpercentage of P. multocida isolation (13.1%), followed by Nasal Swabs (5%). These isolates were confirmed microscopically to be P. multocida bystaining with Gram's and Leishman's stains, then biochemically by traditional test likeindole, catalase, oxidase, urease, citrate utilization, sugar fermentation and gelatinliquefaction tests. Also, the results were confirmed using commercial test API 20E andPCR. For common gene and capsular serotyping of P. multocida by using multiplex capsular PCR typing system, a total of 9 representative isolates weretested and 5 isolates were positive for common gene of P. multocida and positive for capsular type E. Positive P. multocida isolates were tested for presence of virulence factors by multiplex PCR for virulence genotyping which represented by virulence genes (TbpA, nanH, ompH and ptfA) which revealed 100% percentages of Tbpa and nanH in lung tissues and nasal swabs in cattle and buffaloes isolates, the presence of ptfA gene in high percentages in lung tissues in buffalo more than in nasal swabs.