article · Current Protocols
Abstract Reticulocytes are a group of immature red blood cells (RBCs) produced in the bone marrow and released into the bloodstream where they mature into RBCs within 1 to 2 days. They play a crucial role in assessing bone health and many hematological diseases. Isolating viable reticulocytes for use in single‐cell transcriptomics has been a challenge due to their similarity to mature RBCs. Their low concentration and continuous maturation to RBCs with time also poses a great challenge. In this article, we optimized a method for enrichment of CD71 + reticulocytes from whole blood, with a high yield and viability for downstream studies. © 2025 Wiley Periodicals LLC. Basic Protocol 1 : Blood tissue preparation and reticulocyte enrichment Basic Protocol 2 : Visualization and determination of cell viability Alternate Protocol : Counting cells using a hemocytometer
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DOI: 10.1002/cpz1.70203
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