article · International journal of medicinal mushrooms
Research into the medicinal mushroom Lentinus edodes demonstrates that combining strain mutation with computational optimisation significantly enhances exopolysaccharide yields during submerged fermentation. Treating the wild mushroom strain with ultraviolet irradiation and acridine orange increased yields from an initial 1.044 grams per litre to 2.783 and 5.548 grams per litre, respectively. Further fermentation optimisation using a genetic algorithm coupled with an artificial neural network substantially raised productivity to 23.21 grams per litre. The resulting exopolysaccharides showed antioxidant properties, achieving 45.40 to 88.90 percent DPPH radical scavenging activity. Additionally, the compounds inhibited the growth of the pathogenic bacteria Escherichia coli and Klebsiella pneumoniae. This multistep approach highlights how physical-chemical mutagenesis and media optimisation can improve bioactive compound production from medicinal fungi.
Enhancing yields of functional compounds from fungi is often limited by low natural production rates. By combining physical or chemical mutations with artificial intelligence modelling, bioactivity yields can be multiplied many times over. The produced substances exhibit potent antioxidant and antibacterial properties, offering potential value for developing natural therapeutics and functional formulations.
The findings are relevant to industrial biotechnology, biopharmaceutical, and nutraceutical developers seeking efficient methods to culture bioactive mushroom compounds. The exopolysaccharide could serve as an ingredient for antioxidant or antibacterial formulations. The research appears to be at an applied laboratory stage, having demonstrated successful submerged fermentation optimisation and bioactivity testing, but requiring further scale-up and validation before industrial adoption.
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Exopolysaccharide (EPS) production by a strain of Lentinus edodes was studied via the effects of treatments with ultraviolet (UV) irradiation and acridine orange. Furthermore, optimization of EPS production was studied using a genetic algorithm coupled with an artificial neural network in submerged fermentation. Exposure to irradiation and acridine orange resulted in improved EPS production (2.783 and 5.548 g/L, respectively) when compared with the wild strain (1.044 g/L), whereas optimization led to improved productivity (23.21 g/L). The EPS produced by various strains also demonstrated good DPPH scavenging activities of 45.40-88.90%, and also inhibited the growth of Escherichia coli and Klebsiella pneumoniae. This study shows that multistep optimization schemes involving physical-chemical mutation and media optimization can be an attractive strategy for improving the yield of bioactives from medicinal mushrooms. To the best of our knowledge, this report presents the first reference of a multistep approach to optimizing EPS production in L. edodes.
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DOI: 10.1615/intjmedmushrooms.v18.i7.20
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