Molecular Genetic Variations of Some Rabbit Breeds Using Small Mitochondrial rRNA Sequences

Abstract

With the help of small mitochondrial rRNA (12S rRNA), the genetic diversity of five different rabbit breeds in Egypt was estimated in the current study. Slight difference was found between 12S rRNA sequences of the five different rabbit breeds where, the sequence size was 923 bp in four breeds and 924 bp in New Zealand White breed. The final alignments consisted of 924 bp., of them 913 conserved sites. The accession numbers for these sequences in GenBank/NCBI were (OR210201-OR210205). The nucleotide frequency average was (A) 36%, (T) 23.68%, (C) 22.36%, and (G) 17.96%. All breeds had an average A+T rating of 59.68%. The genetic distance values amongst breeds extended from 0.000 to 0.0033. The (0.0033) percentage was observed amongst the breed New Zealand White and REX breed. As well as high genetic variations were observed between New Zealand White breed and the rest breeds. The information provided by the 12S rRNA sequences demonstrated that the New Zealand White breed was genetically unique from the other breeds. The investigation's findings validate the suitability and qualification of 12S rRNA for observing breed-specific genetic variation in rabbits.The generation of genetic maintenance and amelioration strategies for Egyptian rabbit genetic resources may benefit from this data in the future.