Mitigating Lipopolysaccharide‐Induced Hepatorenal Injury: The Role of Chrysin‐Loaded Poly (Lactic‐Co‐Glycolic Acid) Nanoparticle in Modulating Oxidative Stress and Inflammation

Abstract

Chrysin (CHR), a naturally occurring flavone recognized for its anti-inflammatory and antioxidant activities, exhibits medicinal properties; nevertheless, the clinical use is constrained by inadequate absorption and limited bioavailability. This research employed poly-lactic-co-glycolic acid (PLGA) as a nanodrug carrier to enhance the bioavailability, solubility, and effectiveness of CHR in protecting mice against lipopolysaccharide (LPS)-acute liver and kidney damage. CHR-loaded PLGA nanoparticles (CHR-NPs) were synthesized and analyzed. Mice were allocated into six groups (n = 8/group): control, LPS, Dexa (5 mg/kg), PLGA (50 mg/kg), CHR (50 mg/kg), and CHR-NP (50 mg/kg). CHR-NP improved liver and kidney function, alleviated histopathological abnormalities, and elevated enzymatic and non-enzymatic antioxidants while reducing iron deposition, lipid peroxidation, and proinflammatory mediators. Furthermore, the Keap1/Nrf2/HO-1 signaling pathway was upregulated after oral administration of CHR-NP. Through activating the Keap1/Nrf2/HO-1 signaling pathways, our data demonstrate that CHR-NP significantly alleviated acute liver and kidney injury by inhibiting oxidative stress and inflammation.