article · HortScience
Research has optimised a micropropagation protocol for lacy tree philodendron using shoot tip explants. Evaluating growth regulators, nutrient concentrations, light levels, and culture types identified key conditions for efficient regeneration. Supplementing Murashige and Skoog medium with 1 milligram per litre of 6-benzylaminopurine and 0.5 milligrams per litre of indole-3-butyric acid achieved the highest multiplication rate of 11.4 shoots per explant. Reduced salt strength, sucrose levels above 30 grams per litre, and high light intensity hindered shoot production. Gelled medium favoured shoot proliferation, whereas liquid or bioreactor systems enhanced shoot growth. For rooting, adding 1 to 2 milligrams per litre of naphthalene acetic acid achieved 100 percent rooting success alongside superior root weight. All resulting plantlets were successfully acclimatised with a 100 percent survival rate, showing true-to-type morphology.
Lacy tree philodendron is an ornamental plant, but conventional propagation can be slow or inconsistent. Establishing a refined, reliable tissue culture protocol enables rapid, large-scale production of uniform, healthy plants. Defining the exact nutrient, hormone, and lighting requirements ensures high plantlet survival and consistent quality, helping to overcome production bottlenecks in commercial plant production.
This applied protocol provides an actionable tissue culture recipe for commercial nurseries and ornamental micropropagation laboratories. Because the method achieves high multiplication, complete rooting, and 100 percent acclimatisation survival with true-to-type morphology, it appears ready for testing and adoption in commercial facilities. The findings regarding bioreactor cultures versus gelled media also offer practical guidance for scaling up industrial propagation pipelines.
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The present study aimed to optimize the micropropagation of lacy tree philodendron using shoot tip explants. Axillary shoot regeneration was investigated in Murashige and Skoog (MS) medium with different types and concentrations of plant growth regulators, varied levels of MS medium salt strength, sucrose concentration, and light intensity and culture type. Adding 6-benzylaminopurine (BAP; 1 mg·L −1 ) significantly increased shoot multiplication compared with other cytokinins, and the combination of cytokinins and auxins [indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA)], yielded more shoots than cytokinins alone, with the greatest number of axillary shoots (11.4 per explant) obtained using both BAP (1 mg·L −1 ) and IBA (0.5 mg·L −1 ). In addition, the use of half-strength salt concentrations significantly reduced shoot multiplication, and high sucrose concentrations (>30 g·L −1 ) reduced explant growth. High light intensity also reduced shoot multiplication and growth, owing to photoinhibition, and shoot multiplication was more efficient in gelled culture, whereas shoot growth was greater in liquid/bioreactor culture. The best rooting success (100%) and greatest root number and fresh weight were obtained using MS medium supplemented with NAA (1–2 mg·L −1 ). The resulting plantlets were successfully acclimatized, with a survival rate of 100%, and were morphologically similar to the mother plant.
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DOI: 10.21273/hortsci14612-19
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