Microbial Chitinases — Production, Characterization, Purification and their Biotechnological and Therapeutic Applications: An Integrated Review

Abstract

Chitin is the second most abundant natural polysaccharide after cellulose and consists of N-acetyl-D-glucosamine units linked by β-1,4-glycosidic bonds. In nature, chitin does not accumulate due to the synergistic action of chitinolytic enzymes. Based on their catalytic domains, chitinases are classified into glycosyl hydrolase families GH18 and GH19. They are widely produced by bacteria and filamentous fungi. Different types of chitinolytic enzymes, including endochitinases, exo-acting enzymes, and N-acetylglucosaminidases, have been reported to exhibit antimicrobial and insecticidal activities, making them valuable tools for controlling phytopathogenic fungi and insect pests. Chitin degradation generates chitooligosaccharides (COS), which possess diverse biological properties such as antimicrobial, antioxidant, anti-inflammatory, and antitumor activities, contributing to improved human health. Microbial chitinases are also applied in several industrial and environmental processes, including protoplast formation, single-cell protein production, and dye removal. Advances in recombinant expression and genetic engineering have enhanced chitinase production, stability, and catalytic efficiency. Moreover, recombinant chitinases have been successfully utilized in biocontrol strategies and in developing transgenic plants with increased resistance to phytopathogens. This review highlights the broad agricultural, industrial, and biomedical applications of chitinases and their crucial role in promoting environmental sustainability and advancing bio-based industrial processes.