article · Natural Product Research
<i>Lagenaria siceraria</i>, a medicinally valuable Cucurbitaceae species, remains understudied for plant tissue culture (PTC) applications. This study explored suspension cultures treated with 2,4 Dichlorophenoxy acetic acid (2,4-D), Indole-3-acetic acid (IAA), α-naphthyl acetic acid (NAA), Gibberllic acid (GA) and their combinations to enhance secondary metabolite production. Metabolic profiling <i>via</i> UPLC-MS/MS identified 81compounds, including triterpenes, amino acids, flavonoids, phenolic acids, fatty acids and organic acids. Growth regulators as NAA and GA succeeded in upregulating organic acids by 15 folds compared to leaf extract. While 2,4 D showed an increase of 7, 5 and 4.5 folds in expression of triterpenoids, phenolic acids and amino acids, respectively. Combinations of 2,4 D and NAA induced 4 folds rise in fatty acid concentration compared to the leaf extract. Multivariate analyses and heat maps revealed distinct clustering between wild leaf extracts and hormone-treated cultures. VIP scores highlighted uridine, syringaldehyde, myricetin, isovaleric acid as key discriminants.
This page summarises published work. The authoritative version sits with the publisher.
DOI: 10.1080/14786419.2025.2581221
Is something wrong with this record? Report it or request removal.
Discussion
Have you built on this work, tried to replicate it, or seen it applied in practice? Share what you know. Verified researchers and MARATTO™ domain experts can open a discussion, and any member can reply. Contributions are reviewed before they appear.
No discussion yet. Open the first thread.
New to MARATTO™? Create a free account.
