Isolation and identification of multi-drug resistant Klebsiella pneumoniae from patients of some hospitals in Dakahlia Governorate, Egypt

Abstract

Klebsiella pneumoniae poses a significant risk to public health as it is a crucial human pathogen responsible for opportunistic nosocomial and community-acquired infections. One of the major concerns is the rising prevalence of multidrug-resistant strains. 102 clinical samples of urine, blood and wound swabs were collected from diabetic, urinary and respiratory tract infections patients who were admitted to Talkha, Nabrouh Central Hospitals and Sandoub Health Insurance Hospital in Dakahlia Governorate. 25 of 102 isolates resembling Klebsiella pneumoniae were identified by conventional biochemical methods. An antibiotic sensitivity test was conducted on isolates of Klebsiella pneumoniae using 9 antibiotic disks. The results revealed that 92% of the isolates exhibited resistance to Piperacillin, while 84% demonstrated resistance to both Ceftriaxone and Ceftazidime. Additionally, a majority of the isolates, accounting for 60%, displayed resistance to Ampicillin-Sulbactam and Cefepime.Top of Form 16s rRNA analysis was performed to isolate KP26 that which was the most resistant to all antibiotics. It was similar to Klebsiella pneumoniae strain PF-4 according to NCBI-BLAST and its alignment in the neighbor-joining tree results. The study findings emphasized a notable threat to human health, as the preponderance of isolates exhibited resistance to multiple antibiotics.