Isolation and diagnosis of multi-drug resistance antibiotics bacteria in diabetic foot infections of Mansoura University Hospitals

Abstract

One of the most frequent consequences of diabetes is diabetic foot infection (DFI). The antibiotic susceptibility of the pathogens causing DFI varies depending on the region. The infections that are most likely to be present should therefore be the basis for empirical antibiotic therapy. The objective is to enable clinicians in our community select the most appropriate empirical antibiotic for DFI by identifying the common aerobic bacteria that cause DFI and determining their antibiotic susceptibility. Methods: 200 diabetic foot ulcers (DFUs) had swabs taken from males and females admitted to Mansoura University Hospitals. Testing for antibiotic susceptibility and bacterial identification by culturing, morphology and biochemical tests of all isolated bacterial cultures. Results: A total of 49 clinical isolates of the four different microorganisms were cultured and sensitivity tested towards 14 antibiotics [(Cefazolin, Ceftazidime, Cefotaxime, Ciprofloxacin, Meropenem, CEP, Ampicillin, Amoxicillin + clavulanic acid, Piperacillin, Trimethoprim, RTE, SAM and Amikacin)] to determine the extent of the bacteria’s resistance to antibiotics. The results in the current study revealed that there were statistically significant difference between strain resistance to ceftazidime , As the results showed that there was a statistically significant difference between strain resistance to CEP, also registered a statistically significant difference between strain resistance to Amoxicillin + clavulanic acid, Likewise for the resistance to RTE, SAM and Amikacin, The study also showed that Staphylococcus bacteria had the highest rate of resistance to different tested antibiotics. Conclusions: S. aureus is the most common cause of diabetic foot infection at our study, at the same time, it is the most resistant to antibiotics.