Identification, Serological and Molecular Identification of Coliform Bacteria Recovered from Bovine Mastitis

Abstract

Aim/Background: Mastitis is one of the serious disease and critical problem in the dairyindustry worldwide. This study aimed to determine the prevalence of coliform bacteria,particularly E. coli, associated with mastitis in dairy herds. In addition, serologicalidentification and molecular detection for E. coli strains from bovine mastitis.Methods: A total of 400 cows were inspected for clinical and subclinical mastitis, and 100samples (64 and 36, respectively) from these conditions were collected for bacteriologicalidentification before being subjected to serological and molecular analysis.Results: Clinical and subclinical mastitis were prevalent; 16% and 9%, respectively.Bacteriological examination revealed that E. coli was the most prevalent bacteria 16isolates (19.51%) followed by Klebsiella spp 8 (9.76%), Citrobacter 4 (8.16%), andEnterobacter aerogens 2 (4.08%). Regarding to the serological results, E. coli isolatesclassified into EHEC which was the most identified serotype followed by EPEC and ETECserotypes. While, for Klebsiella spp., Klebsiell aoxytoca and Klebsiella pneumonia were serologicallyidentified. The molecular detection some of the virulence genes by PCR approach amongE.coli isolates reported that all E. coli strains carried the fimH (100%). Meanwhile, othervirulence genes sxt1 and eaeA gene didn’t detected in any tested E. coli isolates.Conclusion: Coliform bacteria were identified as major bacterial pathogens involved inbovine clinical and subclinical mastitis.