Healing Effect of Topically Applied Nano-Curcumin on Buccal Traumatic Ulcer in Albino Rats (Histological and Immunohistochemical study)

Abstract

BackgroundCurcumin have numerous biological advantages, such as antioxidant, anti-inflammatory and antimicrobial properties. However, its practical application is hindered by low solubility and short plasma half-life. Therefore, this study seeks to evaluate the impact of topically administered Nano-curcumin on the healing of induced traumatic ulcers in the buccal mucosa of albino rats.Material and methods:Fifty-four adult albino rats were assigned to five groups. The negative control group (n=12) received no ulcer induction or treatment and was euthanized at two time points: on the 7th day and the 14th day. 2-Positive control group: (n=6), buccal traumatic ulcers were induced, and the rats were euthanized after 2 hours. 3-Self-healing group: (n=12), induction of traumatic ulcer in buccal mucosa of rats, then euthanization after 7th and 14th days (6 rats for each intervals) for self-healing evaluation. 4-Curcumin treated group: (n=12), induction of buccal traumatic ulcer, curcumin gel was topically applied on ulcer twice daily, euthanization after 7th and 14th days (6 rats for each intervals). Nano-Curcumin group: (n=12), induction of buccal traumatic ulcer, Nano-curcumin emulsion was topically applied on ulcer twice daily, euthanization after 7th and 14th days (6 rats for each intervals). Ulcer healing was evaluated by Histopathological and immuno-histochemical assessments for TGF-βII and E-Cadherin.Results: Nano-Curcumin treated group indicating potential for ulcer healing histopathologically and immuno-histochemically compared to other studying groups in albino rates.Conclusion: Nano-curcumin represent effective alternative to conventional medication and has superiority on curcumin regarding the acceleration of ulcer healing due to better solubility and enhanced Nano-properties.