GENETICALLY IMPROVEMENT OF SOME PERFORMANCE TRAITS OF EGYPTION LOCAL CHICKEN BY TRANSFERRING GROWTH HORMONE GENE FROM BROILER

Abstract

The present study was aimed to investigate isolation, cloning and sequencing of chicken Growth hormone gene (cGH) from chicken (cobb 500 broilers) then transfer chick-en Growth hormone gene by two methods the first one gene transfer using sperm-mediated gene transfer (SMGT) technique and the second method gene transfer using bioresonance (Bio) to local chicken strain (Bandarah). Total RNA was extracted from chicken liver tissue and the cDNA was successfully prepared. The recombinant cDNA with the Gen Bank ref-erence sequence accession number: LC441152.1. The body weight at hatch and at 4 weeks of age increased by 3.32 g and 8.09 for SMGT method and by 1.27 and 5.22 for Bio meth-od respectively. The SMGT method was increased the body weight at 12 wk of age by 60.17 g and 7.96 g for males and females respectively. The growth rate during 0-4 weeks of age was 131.64%, 130.68 and 132.35%, for SMGT, Bio and control respectively. In conclusion cGH Gene successfully isolate, molecular cloning from Cobb 500 and transfer-ring by two methods SMGT and Bio to production transgenic chickens of a local strain in Egypt .SMGT is an efficient method that will hopefully facilitate the implementation of strategies for securing the benefits that can be expected to arise from the introduction of transgenic chicken, Bio open important new perspectives in the field of animal trans genesis would be more rapid, with quick and effective delivery of genes to target tissues. Chicken cGH gene was effect in all performance traits and moved from the first generation to the second with the same shape and increased the effect.