EVALUATION OF MODIFIED IN SITU PINHOLE TECHNIQUE FOR CASTRATION IN HORSES

Abstract

A modified in Situ Pinhole technique (MPH) for castration in the horse was evaluated in a clinical trial. Plasma testosterone concentrations after challenge with human chorionic gonadotrophin (hCG), and histopathological changes of the testicular tissue were also assessed. Five stallions, aged 12-14 years, were castrated using MPH technique under general anaesthesia. The spermatic cords were double clamped and ligated. Both testes were left in situ. Testosterone concentrations were measured before, 2 months after castration, and immediately 1h and 24h after injection of 10,000 IU of hCG. Both testes from each castrate were collected at 2 months for histopathologic examination. MPH castration was successfully achieved. Moderated scrotal and preputial swelling was the only experienced short term complication. Testosterone concentrations were significantly lower than basal pre-operative levels at 2 months after castration, and did not respond to hCG. On histopathology, hyalinization of the seminiferous tubules and loss of testicular interstitial tissue were indicative of complete avascular necrosis. This modified primary closure castration technique of stallion is a simple practical method, with minimal postoperative complications; and could be safely advocated as an alternative to the traditional castration techniques allowing for second intention healing of scrotal wounds.