Evaluation of In-vitro Angiotensin Converting Enzyme (ACE) Property of (Balanites aeqyptiaca. Del) Meal, Defatted Meal, Concentrate and Hydrolysates

Abstract

Edible seed proteins stand greater chance to manage chronic diseases.Balnites aegyptiaca seed is widely natural across Africa and in Asian countries.This desert three seed nut has potential nutritional properties which are been utilized at home nowadays.This study was to determine the in vitro ACE potentials of Aduwa protein meals and hydrolysate.Aduwa seeds were toasted at 70 °C, milled and oil extracted to make APM.The meal was defatted made into concentrate and finally into hydrolysates.The hydrolysate was hydrolyzed by using pepsin, pancreatin, pepsin and pancreatin combines protease.The samples were subjected to different inhibitory concentration EC 5 , EC 10 and EC 20 invitro angiotensin converting enzyme activities.The result revealed that Aduwa meal protein and hydrolysate has the potential to reduce blood pressure.The resolved meals exhibited greater angiotensin converting enzyme activities at five inhibitory concentrations (EC 5 ) respectively, for defatted meal was (5%), concentrate (3%) pancreatin hydrolysate (4%), pepsin hydrolysate (2%) and combined hydrolysate (2%).Inhibition concentration at EC10 exhibited significant high angiotensin converting enzyme activities value in defatted meal and pancreatin hydrolysate when compared with the referral control.The results suggested that defatted meal, pancreatin hydrolysate at EC 10 and pepsin hydrolysate at EC 5 and EC 20 respectively had better invitro angiotensin converting enzyme inhibitory potentials, hence Aduwa seed protein bioactive has inhibitory potentials to act as antihypertensive agents that could be utilized as ingredients for the development of novel functional foods and nutraceuticals in future.