Evaluating the pharmacological activities of <i>Aloe perryi</i>–Silver nanoparticles induced apoptosis against colon cancer cells (<scp>HCT</scp>‐116)

Abstract

<i>Aloe perryi</i> has been studied and possesses several activities, including antibacterial, antiparasitic, and anticancer properties. In this study, <i>A. perryi</i> was used as a reducing agent of silver ions into silver nanoparticles. <i>Aloe perryi-</i>silver nanoparticles (APS-NPs) were characterized and evaluated using characterization techniques. However, the antioxidative, antibacterial, and anticancer assays were studied to evaluate the pharmacological activities of APS-NPs. APS-NPs were developed and changed to dark brown and the maximum absorption was 442 nm. SEM (5-583 nm), TEM (4-110 nm), XRD (21.84 nm), and zeta potential analysis (63.39 nm) revealed that the APS-NPs were nano-sized, and the APS-NPs had a cubic crystalline structure, according to the XRD results. FTIR analysis suggested that functional groups of <i>A. perryi</i> metabolites were involved in forming APS-NPs. The zeta potential indicated that the APS-NPs were negatively charged (-32 mV), suggesting good stability. APS-NPs showed significant antioxidative stress activity by reducing DPPH-free radicles in a dose-dependent manner. APS-NPs-induced antibacterial effect against <i>Staphylococcus aureus</i> (<i>S. aureus</i>), <i>Escherichia coli</i> (<i>E. coli</i>), and <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>). APS-NPs reduced the cell viability and cell migration of the human colon tumor cell line (HCT 116) compared with controls, indicating that APS-NPs could play a role in reducing metastasis and inducing cell apoptosis against colon cancer. In conclusion, the nanoparticle synthesis from <i>A. perryi</i> extract demonstrated excellent antioxidant, antibacterial, and anticancer activities, thus suggesting that our APS-NPs have the potential to be used as antioxidative and antibacterial in food and pharmaceutical industries.