article · Natural Product Research
Inhibitory effect of the tested plant oils and their nanoformulation against trypsin and chymotrypsin enzyme extracted from <i>Rhynchophorus ferrugineus</i>, was studies in <i>in vivo and in vitro. In vivo</i> inhibition, the reduction in the trypsin specific activity caused by the bulk oils was greater than that of chymotrypsin enzyme. Compared to control nanoformulation caused significant reduction in the specific activity of trypsin enzyme; however, there were no inhibition effect observed on chymotrypsin enzyme. <i>In vitro</i> bulk oils showed very low inhibitory effect against trypsin enzyme, while they showed strong inhibition activity against chymotrypsin with recording for ginger nanoformulation (half-maximal inhibitory concentration (IC<sub>50</sub>) =6.83 μg/ml), purple nutsedge (7.632 μg/ml), garden cress (11.52 μg/ml) and castor (16.82 μg/ml), nanoformulation of these oils showed uncompetitive type of inhibition as the <i>Vmax</i> and <i>Km</i> values changed compared to control. In conclusion, nanoformulation of the tested plant oils could be used in the integrated pest management (IPM) programs of the red palm weevil (RPW).
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DOI: 10.1080/14786419.2024.2302315
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