Environmental culture optimization for maximizing alkaline protease production by local Bacillus subtillis isolated from vegetables water waste

Abstract

Alkaline protease have tremendous applications in different industries. Therefor in this study, we reported the production of thermostable alkaline protease from thermotolerant alkaliphilic bacterial isolates. This bacteria was isolated from vegetable water waste, screened proteolytically showing the maximum zone of inhibition and identified as Bacillus subtilies based on 16S rDNA sequencing. Maximum level of protease production was founded at 40 0c of fermentation in alkaline skimmed milk nutrient broth media. The optimum incubation period, size of inoculum and initial pH of the media for maximum production of alkaline protease were 48h, 2ml% and 8 respectively at shaking conditions of 150 rpm. Where the enzyme activity was reported as following: 0.470 U/ml for optimum pH, 0.612 U/ml for optimum incubation period, 0.504 U/ml for optimum incubation temperature, 0.351 U/ml for best inoculum size and 0.452 U/ml for shaking conditions. Thus, such additions can influence alkaline protease production and improve their use in various applications of industry.