article · American Journal of Molecular Biology
Ten Egyptian barley genotypes, comprising two commercial varieties and eight breeding lines, were evaluated under normal and salinity stress field conditions to identify salt-tolerant lines using a susceptibility index. The genotypes Giza 123, Line-1, Line-5, Line-6, and Line-8 demonstrated high tolerance indices under saline conditions. To assess genetic variation and establish fingerprinting profiles, the genotypes were analysed using seven SSR markers alongside a novel approach combining up to three SCoT primers in single reactions. The Triple-SCoT strategy achieved a polymorphism rate of 94.44 percent, compared to 90.91 percent for SSR markers, and generated a higher average number of polymorphic fragments per primer as well as more genotype-specific markers. Cluster analysis confirmed that the genetic groupings produced by Triple-SCoT closely aligned with the established SSR marker patterns.
Soil salinity presents a significant challenge to agricultural productivity and crop yields. Identifying salt-tolerant crop varieties and validating efficient, highly accurate molecular screening techniques helps plant breeders select resilient genetic lines more effectively, supporting efforts to sustain food production in salt-affected environments.
This research provides applied molecular screening tools and identifies specific salt-tolerant barley lines that can be directly utilised by plant breeders and agricultural research organisations. The Triple-SCoT method serves as an efficient fingerprinting technique for genetic characterisation. The work represents applied, tested research ready for integration into ongoing barley breeding programmes, though the abstract does not indicate immediate commercial product release.
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Ten Egyptian barley genotypes (2 commercial varieties and 8 breeding lines) were cultivated under normal condition at the Experimental Farm of Sakha Agricultural Research station and exposed to salinity stress condition at the Experimental Farm of El-hosainia plain Agricultural Research station, Elsharkia Governorate, Egypt, in an attempt to identify the relative salinity tolerant genotypes. A susceptibility index (SI) was used to estimate the relative stress loss because it accounted for variation in yield potential and stress intensity. Giza 123, Line-1, Line-5, Line-6 and Line-8 genotypes were considered as saline tolerant genotypes on the basis of their highly tolerance indices values. Barley genotypes were characterized by seven SSR markers and three SCoT primers in different combinations to discern the extent of genetic variation and develop a fingerprinting key. Normal SCoT reactions amplify single segments of DNA which are 15- to 19-mer long. A new strategy was used to increase SCoT potential in genetic diversity studies by using two and three different primer combinations per reaction. Amplification products scored a polymorphism percentage of 94.44% for Triple-SCoT and 90.91% for SSR, while the average no. of polymorphic fragments/primer was 17 and 7.14 in the two marker systems, respectively. On the other side, Triple-SCoT exhibited the highest average number of positive and negative genotype-specific markers. The cluster analysis of the studied genotypes using these different marker systems revealed four dendrograms varied in their topology. The dendrogram based on Triple-SCoT data exhibited the closest relationships to those illustrated by SSR dendrogram.
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DOI: 10.4236/ajmb.2017.73010
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