Effects of T-Consciousness Fields on Mouse Oocyte Maturation and Embryo Development Following IVF

Abstract

T-Consciousness Fields (TCFs) with non-material and non-energetic entities have been introduced by Taheri. The influence of TCFs can be investigated through laboratory experiments. In this study, two separate works were conducted to evaluate the effects of TCFs on the in vitro maturation of mouse oocytes (IVM), mitochondrial membrane potential and embryo development through in vitro fertilization (IVF). In the first experiment, after 24 hours, the maturation of oocytes either in the presence or absence of TCFs was observed using an inverted microscope and the mitochondrial membrane potential of MII oocytes was estimated through JC-1-aggregate fluorescence intensity. In the second experiment, the effects of TCFs on the rate of fertilization and 2PN zygotes and the grades of embryos were evaluated. The results showed that the maturation of oocytes under TCFs treatment increased by an average of 33% compared to the control (p-value<0.05), and the MII oocytes had a higher mitochondrial membrane potential under the influence of TCF1 (p-value<0.05) and TCF3 (p-value<0.01), suggesting their better efficiency. Moreover, TCFs increased the probability of 2PN zygotes by about 12% compared to the control (p-value=0.0090). Furthermore, TCFs-treated samples exhibited a notable 45% reduction in grade BC (p-value<0.05). Additionally, the percentage of grades A and B of oocytes was more than two times (p-value <0.05) higher than the control. In conclusion, these results provide preliminary evidence of the positive effects of TCFs treatment on fertilization and pave the way for further research in this area.