article · Zenodo (CERN European Organization for Nuclear Research)
This study evaluated the effects of an ethanol extract from Rauwolfia vomitoria leaves on lipid levels, oxidative stress markers, and brain tissue changes in rats exposed to the chemotherapy drug cisplatin. Cisplatin is widely used to treat cancer but can cause damaging oxidative stress and neurological side effects. Rats given cisplatin showed altered lipid profiles, minor increases in oxidative markers, and structural damage to the cerebellar cortex, including distorted Purkinje cells and vacuolation. Treatment with the plant extract at daily doses of 100 mg/kg or 200 mg/kg over 20 days altered lipid concentrations, raised antioxidant activity, and considerably improved cerebellar histology. Overall, the plant extract reduced cellular damage, enhanced antioxidant defences, and displayed protective properties against cisplatin-induced neurotoxicity in the animal model.
Cisplatin is an effective chemotherapy drug, but its neurological toxicity poses major challenges for cancer patients. Demonstrating that a plant extract can protect brain tissue and improve antioxidant capacity in animal models helps researchers identify natural compounds that may ultimately reduce the harmful side effects of standard cancer therapies.
This research is at an early experimental stage using an animal model. It could eventually inform the development of botanical adjuvants or protective therapeutics for pharmaceutical companies seeking to mitigate chemotherapy-induced neurotoxicity. Significant preclinical optimisation, toxicology studies, and human clinical trials would be required before any commercial or therapeutic application can be realised.
AI-generated from the published abstract. Always read the original work before citing.
ABSTRACT <em>Rauwolfia vomitoria </em>has been observed to ameliorate and prevent cisplatin-induced neural loss due to its antioxidant and anti-inflammatory properties. To investigate the effects of ethanol extract of <em>R. vomitoria </em>(RV) leaf on lipid profile, oxidative stress biomarkers, and the cerebellar histology using cisplatin-induced oxidative stress in Wistar albino rats. Thirty-six (36) rats were randomly shared into four groups (<em>n</em>=9). Group1 (positive control) received drinking water and rats feed. Group 2 (negative control) received cisplatin (5mg/kg body weight) via intraperitoneal (IP) injection. Group 3 received cisplatin (5mg/kg body weight) and 100mg/kg/day (RV). Group 4 received cisplatin (5mg/kg body weight) and 200mg/kg/day (RV). RV was given for 20 days via oral gavage while the single dose of cisplatin was given intraperitoneally on the first day. On day 21, lipid profile was analyzed, oxidative stress biomarkers activities were determined, and the cerebellum was prepared using Haematoxylin and Eosin (H&E) and Cresyl Violet (CV) staining techniques. Cisplatin elevated total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), and low density Cisplatin is a platinum-based chemotherapy drug used to treat various types of cancers. lipoprotein (LDL) (<em>p</em>>0.05). The TC, HDL, and LDL in the RV-treated groups reduced slightly (<em>p</em>>0.05), while TG was further elevated (<em>p</em>>0.05). The cisplatin group had an insignificant rise (<em>p</em>>0.05) in lipid peroxidation malondialdehyde, glutathione, catalase, and superoxide dismutase. RV further elevated these values. Cisplatin distorted the cerebellar cortex histology by causing altered Purkinje cell bodies, infiltration of the pyknotic Purkinje cells into the granular layer, fatty changes and vacuolation. The cerebellar histology of rats in groups 3 and 4 showed considerable improvement. The administration of ethanol extract of <em>Rauwolfia vomitoria</em> significantly suppressed oxidative stress, increased antioxidative capacity, ameliorated the histological alterations of the cerebellum, and demonstrated neuroprotective ability against cisplatin-induced neurotoxicity. <strong>Keywords</strong>: <em>Rauwolfia vomitoria, </em>cisplatin, oxidative stress, lipid profile, cerebellar histology, neurotoxicity
This page summarises published work. The authoritative version sits with the publisher.
DOI: 10.5281/zenodo.8042521
Is something wrong with this record? Report it or request removal.
Discussion
Have you built on this work, tried to replicate it, or seen it applied in practice? Share what you know. Verified researchers and MARATTO™ domain experts can open a discussion, and any member can reply. Contributions are reviewed before they appear.
No discussion yet. Open the first thread.
New to MARATTO™? Create a free account.
