Effect of electroinductive conditions on biofilm production capacity of some industrially important bacteria

Abstract

Biofilms are intricate microbial deposits on biotic and abiotic surfaces, with significant medical and biotechnological implications. This study explored biofilm formation by Acetobacter aceti ATCC15973, Pseudomonas aeruginosa ATCC9027, Serratia marcescens ATCC14756, Gluconobacter oxydans ATCC19357, Rhodobacter sphaeroides ATCC17023, and Bacillus subtilis ATCC6633 on wood, glass, steel, PVC, and PET surfaces using qualitative methods. Effects of electrical stimulation (6V, 4.5A), magnetic fields (1000 G), and electromagnetic flux (5 mT) on biofilm formation were assessed via Crystal Violet Binding Assay. G. oxydans ATCC19357 exhibited highest adherence on PVC and wood (2.0145 and 2.402 log cfu/ml, respectively) under electrical stimulation. A. aceti ATCC15973 showed highest adherence on steel, PET, and glass (1.944, 0.9005, and 0.876 log cfu/ml). R. sphaeroides ATCC17023 demonstrated highest adherence on PVC, steel, PET, and glass (1.0895 to 1.7495 log cfu/ml) under magnetic induction; B. subtilis ATCC6633 had highest wood adherence (1.491 log cfu/ml). G. oxydans ATCC19357 showed highest overall adhesion with electromagnetic induction. PVC supported highest biofilm growth (39 %). Biophysical factors varied in enhancing biofilm formation, suggesting potential for bacterial immobilization technologies in bioremediation and industrial fermentation