article · AMB Express
Industrial wastewater often harbours specialised micro-organisms capable of surviving extreme toxicity. Six bacterial strains exhibiting resistance to lead were isolated from industrial wastewater and identified via ribosomal sequencing. Among these, Raoultella planticola FACU 3 displayed the highest maximum tolerance concentration, withstanding lead levels up to 2700 parts per million, alongside notable biosorption and lead uptake capacities. Microscopic analysis confirmed distinctive morphological adaptations in this strain under lead stress. Whole-genome sequencing of this bacterium identified over 5,500 coding sequences, revealing 47 specific genes associated with resistance to various heavy metals including arsenic, zinc, mercury, nickel, silver, chromium, and lead. In addition to extensive heavy metal and antibiotic resistance profiles, genomic evaluation detected several functional genes linked to plant growth promotion, such as phosphate solubilisation and hormone production.
Heavy metal contamination in industrial wastewater poses serious environmental and health hazards. Identifying native bacteria that can naturally survive and absorb high concentrations of toxic elements like lead helps scientists understand how microbes adapt to extreme pollution. This genetic knowledge provides a foundation for biological clean-up methods and multi-functional environmental management.
This early-stage research provides genomic insights and a bacterial candidate relevant to bioremediation and environmental biotechnology developers. The identified strain could potentially be deployed in industrial wastewater treatment or integrated into soil remediation biofertilisers, given its plant growth-promoting genes. However, the findings are currently limited to laboratory-based characterisation and sequencing, meaning substantial field testing and applied development are required before real-world use.
AI-generated from the published abstract. Always read the original work before citing.
Isolation of heavy metals-resistant bacteria from their original habitat is a crucial step in bioremediation. Six lead (Pb) resistant bacterial strains were isolated and identified utilizing 16S rRNA to be Enterobacter ludwigii FACU 4, Shigella flexneri FACU, Microbacterium paraoxydans FACU, Klebsiella pneumoniae subsp. pneumonia FACU, Raoultella planticola FACU 3 and Staphylococcus xylosus FACU. It was determined that all these strains had their Minimum inhibitory concentration (MIC) to be 2500 ppm except R. planticola FACU 3 has a higher maximum tolerance concentration (MTC) up to 2700 ppm. We evaluated the survival of all six strains on lead stress, the efficiency of biosorption and lead uptake. It was found that R. planticola FACU 3 is the highest MTC and S. xylosus FACU was the lowest MTC in this evaluation. Therefore, transmission electron microscopy (TEM) confirmed the difference between the morphological responses of these two strains to lead stress. These findings led to explore more about the genome of R. planticola FACU 3 using illumine Miseq technology. Draft genome sequence analysis revealed the genome size of 5,648,460 bp and G + C content 55.8% and identified 5526 CDS, 75 tRNA and 4 rRNA. Sequencing technology facilitated the identification of about 47 genes related to resistance to many heavy metals including lead, arsenic, zinc, mercury, nickel, silver and chromium of R. planticola FACU 3 strain. Moreover, genome sequencing identified plant growth-promoting genes (PGPGs) including indole acetic acid (IAA) production, phosphate solubilization, phenazine production, trehalose metabolism and 4-hydroxybenzoate production genes and a lot of antibiotic-resistant genes.
This page summarises published work. The authoritative version sits with the publisher.
DOI: 10.1186/s13568-023-01519-w
Is something wrong with this record? Report it or request removal.
Discussion
Have you built on this work, tried to replicate it, or seen it applied in practice? Share what you know. Verified researchers and MARATTO™ domain experts can open a discussion, and any member can reply. Contributions are reviewed before they appear.
No discussion yet. Open the first thread.
New to MARATTO™? Create a free account.
