Diversity of Antibiotic-resistant of Tentative Motile Aeromonas Species Isolated From Clarias gariepinus (Burchell 1822) Cultured in Earthen Ponds

Abstract

Background: Aeromonas species is one of the most important causes of diseases in Clarias gariepinus, a public health threat with significant economic losses. Objectives: In this research, the prevalence and variety of Aeromonas species isolated from C. gariepinus cultured in an earthen pond were investigated, as well as the antibiogram and multiple antibiotic resistance index Methods: Aeromonas species were isolated by culture and biochemical test and confirmed using a Microbact 24E kit. The antibiotic susceptibility to 10 antibiotics was determined using the Kirby-Bauer disk diffusion method. Results: Aeromonas species were isolated with a prevalence of 43.1% with 4 different phenospecies with the highest prevalence of 46(24 %) for Aeromonas hydrophila followed by 15(7.8%) for Aeromonas caviae, then 10(5.2%) for Aeromonas veronii sobria, and the least was observed for Aeromonas veronii veronii 6(3.1%). The Aeromonas species showed high resistance to amoxicillin, ampicillin, colistin sulfate, oxytetracycline, trimethoprim/sulfamethoxazole, and penicillin, with varying resistant patterns, and the multiple antibiotic resistance index values ranged between 0.20 and 0.80. Conclusion: There was a diversity of Aeromonas species associated with multiple antibiotic-resistant leading to the wide spread of antimicrobial resistance. Therefore, there is a need to control the use of antibiotics and ensure the effective use of biosecurity and preventive management measures in fish farms.