Dispersive Liquid - Liquid Microextraction for the Determination of Sulfonamide Residues in Egg Samples

Abstract

A dispersive liquid-liquid microextraction combined with high-performance liquid chromatography-diode array detection was developed for the extraction and determination of 15 sulfonamide residues in the egg matrix. The effects of various parameters such as the type, volume, and composition of extraction solvent for sample treatment procedure, the type and volume of disperser solvent, centrifugation time, salting-out effect and solution pH were studied, and optimum conditions were established. Linearity was found in the range of 5.4 – 1 000 μg kg-1 with regression coefficients ranging from 0.9918 - 0.9987. The limit of detection (LOD) and limit of quantification (LOQ) values of the proposed method were in the range of 4.3 - 8.0 μg kg-1 and 12.9 - 24.0 μg kg-1, respectively. Satisfactory intra-day and inter-day precision results in the range of 6.3 - 17.5% and 4.8 - 16.8%, respectively, were achieved. The accuracy of the method was acceptable with percentage recovery in the range of 73 - 108% and %RSD values in the range of 1.1 - 16.5%. The proposed method was applied in chicken egg samples obtained from supermarkets, and findings confirmed that the method is feasible to be used for extraction and determination sulfonamide residues in egg and related complex biological matrices.