Detection of Wheat Scab Spores Using Terahertz Metamaterial Sensor

Abstract

To achieve label-free, highly sensitive, and rapid quantitative detection of spores of wheat scab pathogens, this study developed a flexible terahertz metamaterial perfect absorber based on a composite unit consisting of dual-U-shaped resonators and a central metal rod. The results showed that the metamaterial exhibited near-perfect absorption at two frequencies, 0.53 THz and 2.30 THz, with absorption rates of 99.2% and 99.5%, respectively. A sharp phase shift occurred at the resonance points, enabling significant amplification of weak sensing signals. The refractive index sensitivity was 110 GHz/RIU at low frequencies and 440 GHz/RIU at high frequencies, indicating superior sensing performance in high-frequency modes. Gradient concentration measurements of Fusarium graminearum conidia revealed a good linear relationship between spore concentration and resonance frequency shift (R2 = 0.996). The detection limit was 10 spores/μL, with a detection range covering 0–1000 spores/μL. This approach meets the needs for early detection of trace amounts of pathogens and quantitative analysis throughout the disease cycle. As this technique requires no labeling, is non-invasive, and operates rapidly, it provides an efficient new method for real-time monitoring and intelligent control of wheat scab in fields. It also holds great potential for applying terahertz metamaterials in agricultural biosafety applications.