Comparison of D-dimer levels using coagulometer and immuno-nephelometer in Covid-19

Abstract

Covid is one of the most lethal viruses in the human respiratory system. Previous outbreaks of Covid19 include a severe respiratory problem (SARS) Cov2 and the Middle East (MERS) respiratory syndrome has recently been identified as one of the most serious health risks. D-dimer is a result of fibrin depletion which increases during a large thrombotic dose attributed to the selective activation of fibrinolytic structure. Measuring D-dimer levels is important in identifying and assessing risk of blood clots and related conditions such as deep vein thrombosis (DVT), pulmonary embolism (PE), and disseminated intravascular coagulation (DIC). This research is of great help to scientists working with new and high standard methods such as Immuno-nephelometer and Coagulometer and the diagnostics machines. Plasma D-dimer levels have demonstrated potential as a prognostic indicator for outcomes in COVID-19 patients. This research aimed to compare D-dimer levels among Covid-19 infected patients at Dr. Soetomo General Academic Hospital using a Coagulometer with an Immuno-Nephelometer. Chest X-rays were performed to confirm active Covid-19 infections and blood samples were collected and centrifuged at 1000 rpm for 15 minutes to get plasma. The plasmas were analyzed using immune-nephelometer and coagulometer diagnostic analyzers. The table showed high levels of D-Dimer which were obtained through analyzers which show the mean and median. The examination and the inflammation were worst, and the chest X-ray showed that shortness of breath became severe. This Study is to make a comparison between CS2500 and Architecti1000 by using immune-nephelometer and coagulometer because the data was statistically analyzed by using KAPPA KOHEN analysis. The confidence interval of 95% (α =0.05) was employed and the results were considered statistically significant. The correlation between CS2500 and Architecti1000 were tested by Mann-Whitney Test correlation and correlation coefficient (r) equals to 0.5 was considered as a threshold to decide the degree of correlation between variables. The findings showed a significant difference between in the D- dimers obtained by CS2500 and Architect i1000 (p=0.019). The compared levels of D-dimer obtained by using coagulometer with an immuno-nephelometer in Covid-19 patients were higher D-Dimer Levels as compared to the normal patients. There is also a difference between CS2500 and Architect i1000 by using immuno-nephelometer and coagulometer because Coagulometer is the more accurate method than the Immuno-nephelometer.