Comparative study between the effect of Parkia Biglobosa (JACQ) benth and conventional antibiotics against multiple antibiotic resistant uropathogenic bacteria (MARUB)

Abstract

The purpose of this research work is to compare the effect of convectional antibiotics with medicinal plant Parkia biglobosa nbsp Jacq Benth against multiple antibiotics resistant Uropathogenic bacteria isolates MARUB which was isolated from basically two hospital in Ondo State Nigeria The two hospitals are Gani Fawehinmi Diagnostic center Ondo and Federal Medical Center Owo between July and October fifty two Gram negative bacteria isolated from urine of UTIs patient at the Gani Fawehinmi Medical Diagnostic Centre Ondo and Federal Medical Centre Owo were screened for antibiotic resistance assay The test isolates were identified characterized with Microbact trade E test strip it was observed that Twenty bacterial isolates were resistant to at least out of of the antibiotics tested showing susceptibility to the carbapenems and piperacillin Tazobactam Five isolates were also susceptible to ofloxacin though with narrower diameter zones of inhibition Parkia biglobosa nbsp Jacq Benth s a plant used in traditional medicine for the treatment of various diseases in Nigeria In order to microbiologically appraise some of the traditional and ethno medical uses of Parkia biglobosa this study aimed at evaluating and compare the diameter zone of inhibition antibacterial activities of Parkia biglobosa leaf and stem bark extracts and conventional antibiotic against multiple antibiotic resistant Uropathogenic bacteria MARUB The Parkia biglobosa extracts shows zone of inhibition against Enterobacter agglomerans Proteus mirabilis Pseudomonas aeruginosa Salmonella Subsp B Serratia marcsesens Citrobacter freundii and Klebsiella pneumonia Diameter zones of inhibition range between mm to mm respectively It was observed that Minimum inhibitory concentration MIC of Parkia biglobosa extracts against the multiple resistant Uropathogenic bacteria ranges between mg ml and mg ml for Stem bark and leaf extracts respectively Pseudomonas aeruginosa has a higher value of Minimum inhibitory concentration MIC of Parkia biglobosa of mg ml for Stem bark and mg ml for leaf extracts The major phytochemical constituent of interests such as Polyphenol Cardiac glycosides Alkaloids Tannins Saponin and Flavonoids were found to be present in Parkia biglobosa In comparing the quantitative content of phytochemical of Parkia biglobosa Stem bark extract contains large quantity of phytochemical compare to the leaf extract These constituents are also higher in acetone extract than ethanol extract These results justifies the uses of Parkia biglobosa in traditional medicine and pave the way for further studies in the developing a new drug