article · Journal of Enzyme Inhibition and Medicinal Chemistry
This research evaluated the chemical composition and biological actions of the chloroform fractions from three marine algae species: Cystoseira myrica, Cystoseira trinodis, and Cystoseira tamariscifolia. Analytical profiling via UPLC/ESI/MS identified 19 metabolites in C. myrica, 20 in C. trinodis, and 11 in C. tamariscifolia, predominantly consisting of terpenoids, flavonoids, phenolic acids, and fatty acids. In laboratory testing, C. trinodis displayed the highest antioxidant capacity in both FRAP and DPPH assays, which was linked to its elevated total phenolic and flavonoid content. Furthermore, enzyme inhibition assays revealed that C. trinodis exerted the strongest inhibitory action against COX-1, while also potently inhibiting the digestive enzymes alpha-glucosidase and alpha-amylase. Molecular docking simulations confirmed that the principal identified metabolites bind efficiently into the catalytic sites of these targeted enzymes.
Managing conditions linked to inflammation and metabolic regulation often drives interest in natural compounds with therapeutic potential. By mapping the chemical constituents of brown seaweeds and testing their enzyme interactions, this study identifies Cystoseira trinodis as an active marine source of dual-acting antioxidant and enzyme-inhibiting agents, providing fundamental biochemical data for research into natural health alternatives.
The findings could ultimately inform the development of natural health ingredients or therapeutic leads targeting inflammation and blood sugar management. Relevant users include pharmaceutical developers and nutraceutical manufacturers investigating marine bioactives. As the results are based entirely on in vitro chemical profiling, enzyme assays, and computational docking models, the work represents early-stage discovery and is several development phases away from real-world application.
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This study aims to investigate the phytoconstituents of the chloroform fraction of three Cystoseira spp. namely C. myrica, C. trinodis, and C. tamariscifolia using UPLC/ESI/MS technique. The results revealed the identification of 19, 20 and 11 metabolites in C. myrica, C. trinodis, and C. tamariscifolia, respectively mainly terpenoids, flavonoids, phenolic acids and fatty acids. Also, an in vitro antioxidant study using FRAP and DPPH assays was conducted where the chloroform fraction of C. trinodis displayed the highest antioxidant activity in both assays, which would be attributed to its highest total phenolics and total flavonoids. Besides, the investigation of COX-1, a-glucosidase and a-amylase inhibitory activities were performed. Regarding C. trinodis, it showed the strongest inhibitory activity towards COX-1. Moreover, it showed potent inhibitory activity towards a-glucosidase and a-amylase enzymes. According to the molecular docking studies, the major compounds characterised showed efficient binding to the active sites of the target enzymes.
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DOI: 10.1080/14756366.2023.2292482
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