COMBINED EFFECT OF CURCUMIN AND PIPERINE ON OBESITY-INDUCED HEMATOPOIETIC, HEPATIC, AND RENAL DYSFUNCTION IN MALE WISTAR RATS

Abstract

One of the adverse effects of obesity is hematopoietic toxicity and vital organs injury. This research sets out to determine whether or not obese rats might avoid damage to their erythrocytes, liver, and kidneys by taking curcumin “CUR” (100 mg/kg of body weight of the rats) and piperine “PIP” (5 mg/kg of body weight of the rats), via stomach tube, daily, for eight weeks. Alanine and aspartate aminotransferases, alkaline phosphatase, and γ-glutamyl transferase activities, platelet and white blood cell counts, as well as total bilirubin and creatinine level, in the blood rose sharply (P≤0.05) after high-fat diet consumption, but albumin level fell sharply (P≤0.05). Obese rat also showed macrocytic normochromic anemia. Obese rats' livers and kidneys showed several abnormalities upon histological analysis. Livers of the high-fat diet group showed dilated central veins with hemorrhage and dilated vascular sinuses, enlarged hepatocytes with numerous large-vessel fatty livers, activated Kupffer cells, and aggregates of inflammatory cells. Renal cortical sections from the high-fat diet group showed dilated glomeruli, congestion of intertubular vessels, and edematous degeneration of some lining epithelial cells. Supplementation with CUR and PIP alleviated significantly (P≤0.05) obesity-related hematopoietic toxicity, as well as liver and kidney damage. Therefore, these results may recommend daily intake of CUR and PIP to prevent obesity-related health risks and liver and kidney toxicity.