Biosynthesis of Chitosan Nanocomposite with Myrrh-Mediated Nanosilver for Controlling Skin Pathogenic Microbes

Abstract

Background: The usages of biosynthesized nanomaterials for microbial pathogens’ fighting have numerous rationales and effectiveness. Skin microbes could acquire drug-resistance that needs innovative approaches for overcoming. Objectives: Phytosynthesized silver nanoparticles (AgNPs) with Commiphora myrrh resin extract (MR) and their nanoconjugates with chitosan nanoparticles (Cht) were fabricated and assessed as potential antimicrobial agents for controlling antibiotic-resistant microbial skin pathogens, Materials and methods: AgNPs biosynthesis was achieved within MR solution and they were composited with Cht. The syntheses of nanomaterials were validated using infrared spectroscopy and electron microscopy and they were loaded onto cotton textiles, then all fabricated nanomaterials/textiles were assessed for inhibiting skin pathogens Staphylococcus aureus and Candida albicans. Results: Nanomaterials’ characterization appointed the mean size of MR-synthesized AgNPs to be 22.58 nm, whereas the mean diameter of Cht/MR/AgNPs nanocomposites was 130.34 nm and carry +25.9 mV charges. The infrared assessment validated the interactions between the employed materials. The loaded cotton textiles with MR/AgNPs and Cht/MR/AgNPs could effectively inhibit the growth of Staphylococcus aureus and Candida albicans, Cht/MR/AgNPs was the most powerful. The scanning microscopy confirmed the antimicrobial action of Cht/MR/AgNPs toward the skin pathogens; the microbes mostly lysed and deformed within 12 h of exposure to nanocomposites. Conclusions: The Cht/MR/AgNPs nanocomposite provided potent antimicrobial actions toward skin microbial pathogens.